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DSMZ

DSMZ 502 . PSEUDOCLOSTRIDIUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 502 #

ml
Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.20 g 1.198 20.493
MgCl2 x 6 H2O 0.40 g 0.399 1.964
KCl 0.30 g 0.299 4.016
CaCl2 x 2 H2O 0.15 g 0.15 1.018
NH4Cl 0.27 g 0.269 5.038
KH2PO4 0.21 g 0.21 1.54
Na2SO4 0.10 g 0.1 0.703
Trace element solution SL-10 1.00 ml - -
Inulin 5.00 g 4.99 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Yeast extract 0.03 g 0.03 -
Casamino acids
(BD Bacto) 		0.03 g 0.03 -
Na2CO3 1.50 g 1.497 14.124
Na2HPO4 2.66 g 2.655 18.7
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.15 g 0.15 0.623
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except yeast extract, Casamino acids, vitamins, carbonate, hydrogenphosphate and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add yeast extract, Casamino acids, hydrogenphosphate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins are prepared under 100% N2 gas atmosphere and strerilized by filtration. Adjust pH of complete medium to 7.0, if necessay.

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 28.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 4

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Inulin 4.99 -
Na2HPO4 2.655 18.7
Na2CO3 1.497 14.124
NaCl 1.198 20.493
MgCl2 x 6 H2O 0.399 1.964
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Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
oxygen anaerobe 4 strains
temperature thermophilic 4 strains