Loading ...

503. ANAEROBIC FRESHWATER (FWM) MEDIUM

Culture medium recipe

Vol.
×
Go back to medium without modifications
* Modified for Bilophila wadsworthia DSM 11045
beta

Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate.

Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 940.0 ml - -
2 Solution B 1.0 ml - -
3 Solution C 1.0 ml - -
4 Solution D 1.0 ml - -
5 Solution E 30.0 ml - -
6 Solution F x ml - -
7 Solution G 10.0 ml - -
* 1.7 g/l - -
Instructions:
  1. Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B, D and G are autoclaved separately under 100% N2 gas. Solutions C and F are prepared under 100% N2 gas and sterilized by filtration. Solution E is autoclaved under 80% N2 and 20% CO2 gas atmosphere. To complete the medium appropriate amounts of solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.2 - 7.4, if necessary.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.20 g 0.213 1.563
2 0.25 g 0.266 4.972
3 1.00 g 1.064 18.203
4 0.40 g 0.426 2.093
5 0.50 g 0.532 7.135
6 0.15 g 0.16 1.085
7
(0.1% w/v)
0.50 ml 5.3e-4 0.002
8 940.00 ml 998.207 55408.8
14 , might need strain adjustment 0.70 g/l - -
Solution occurs in 1 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace element solution SL-10 1 ml - -
Solution occurs in 1 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Seven vitamins solution 1 ml - -
Solution occurs in 1 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Selenite-tungstate solution 1 ml - -
Solution occurs in 1 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.5 g 50 471.752
2 30.0 ml 998.207 55408.8
Solution occurs in 1 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 , might need strain adjustment 1.50 g/l - -
2 , might need strain adjustment x g/l - -
3 , might need strain adjustment 2.50 g/l - -
4 , might need strain adjustment 1.60 g/l - -
5 , might need strain adjustment 2.00 g/l - -
6 , might need strain adjustment 2.00 g/l - -
7 , might need strain adjustment 1.50 g/l - -
8 , might need strain adjustment 20.00 mg - -
9 , might need strain adjustment x g/l - -
10 , might need strain adjustment x g/l - -
11 , might need strain adjustment x g/l - -
12 , might need strain adjustment 2.00 g/l - -
13 , might need strain adjustment 2.50 g/l - -
14 , might need strain adjustment x g/l - -
15 , might need strain adjustment 3.20 g/l - -
16 , might need strain adjustment x g/l - -
17 , might need strain adjustment 2.66 g/l - -
18 , might need strain adjustment 0.50 g/l - -
19 , might need strain adjustment
(BD BBL)
2.00 g/l - -
20 , might need strain adjustment 2.00 g/l - -
21 , might need strain adjustment 0.60 g/l - -
22 , might need strain adjustment
(0.2% w/v)
10.00 ml/l - -
23 , might need strain adjustment 1.22 g/l - -
Instructions:
  1. Use substrate solution depending on organism (see above).
Solution occurs in 1 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 , adjust if required 0.3 g 30 124.905
2 10.0 ml 998.207 55408.8
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: 7.3
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 33
Strains with modifications: 33
Bilophila wadsworthia DSM 11045

  1.70 g/l NaNO3

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Distilled water 981.227 54466.3
NaNO3 1.7 20.001
Na2CO3 1.5 14.153
NaCl 1 17.111
KCl 0.5 6.707
Show more

Cultivation metadata from 33 strains BacDive

halophily not 5 strains
halophily moderate 1 strain
pH 6 - 8 6 strains
pH 8 - 10 4 strains
pH 4 - 6 3 strains
Show more

Similar media

503b. FWM MEDIUM (MODIFIED) 94 %
728. DESULFOBACCA MEDIUM 94 %
298a. LUPHET1 MEDIUM 94 %
194a. DESULFOTOMACULUM OX39 MEDIUM (XYLENE) 93 %
503d. SELENIIVIBRIO MEDIUM (ARSENATE) 93 %

Latest comments

No comments for this medium.