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507. CARBOXYDOTHERMUS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.33 g 0.326 4.374
2 0.52 g 0.514 2.527
3 0.29 g 0.287 1.949
4 0.33 g 0.326 6.096
5 0.33 g 0.326 2.396
6 Trace element solution SL-11 1.00 ml - -
7
(0.1% w/v)
0.50 ml 4.9e-4 0.002
8 1.00 g 0.988 11.763
9 , might need strain adjustment 0.05 g - -
10 Vitamin solution 10.00 ml - -
11 0.70 g 0.692 2.88
12 1000.00 ml 986.371 54751.8
13 Selenite-tungstate solution , might need strain adjustment 1.00 ml - -
14 , might need strain adjustment 0.20 g/l - -
15 , might need strain adjustment 10.00 g/l - -
16 , might need strain adjustment 2.00 g/l - -
Instructions:
  1. Dissolve ingredients except bicarbonate, yeast extract, vitamins and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add yeast extract, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Neutralize the sodium sulfide with sterile H2SO4 (see medium 29). After completing the medium adjust pH to 6.8-7.0. Inoculated vessels are pressurized with sterile carbon monoxide gas to 2 bar overpressure.
Solution occurs in 22 other media (first defined in medium 722 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.2 g 5.2 13.97
2 1.5 g 1.5 7.545
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 24.0 mg 0.024 0.101
9 36.0 mg 0.036 0.149
10 1000.0 ml 998.207 55408.8
Instructions:
  1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 9
Strains with modifications: 9

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.221 55354.1
NaHCO3 0.988 11.763
Na2S x 9 H2O 0.692 2.88
MgCl2 x 6 H2O 0.514 2.527
NH4Cl 0.326 6.096
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Cultivation metadata from 9 strains BacDive

pH 6 - 8 4 strains
pH 8 - 10 3 strains
pH 4 - 6 2 strains
phyla 2 9 strains
nutrition organoheterotroph 1 strain
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