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507. CARBOXYDOTHERMUS MEDIUM

Culture medium recipe

Vol.
×

M 507 #

ml
Equipment needed: Hungate tubes Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.33 g 0.326 4.374
2 0.52 g 0.514 2.527
3 0.29 g 0.287 1.949
4 0.33 g 0.326 6.096
5 0.33 g 0.326 2.396
6 Trace element solution SL-11 1.00 ml - -
7
(0.1% w/v)
0.50 ml 4.9e-4 0.002
8 1.00 g 0.988 11.763
10 Vitamin solution 10.00 ml - -
11 0.70 g 0.692 2.88
12 1000.00 ml 986.371 54751.8
Instructions:
  1. Dissolve ingredients except bicarbonate, yeast extract, vitamins and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add yeast extract, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Neutralize the sodium sulfide with sterile H2SO4 (see medium 29). After completing the medium adjust pH to 6.8-7.0. Inoculated vessels are pressurized with sterile carbon monoxide gas to 2 bar overpressure.
Solution occurs in 22 other media (first defined in medium 722 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.2 g 5.2 13.97
2 1.5 g 1.5 7.545
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 24.0 mg 0.024 0.101
9 36.0 mg 0.036 0.149
10 1000.0 ml 998.207 55408.8
11 , required for pH adjustment x - -
Instructions:
  1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.

SL 1: Vitamin solution #

1000
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 9
Strains with modifications: 9
507 CARBOXYDOTHERMUS MEDIUM
a CARBOXYDOTHERMUS SIDEROPHILUS MEDIUM
b CARBOXYDOCELLA MANGANICA MEDIUM

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.221 55354.1
NaHCO3 0.988 11.763
Na2S x 9 H2O 0.692 2.88
MgCl2 x 6 H2O 0.514 2.527
NH4Cl 0.326 6.096
KCl 0.326 4.374
KH2PO4 0.326 2.396
CaCl2 x 2 H2O 0.287 1.949
Na2-EDTA x 2 H2O 0.005 0.014
FeCl2 x 4 H2O 0.001 0.007
Na-resazurin 4.9e-4 0.002
CoCl2 x 6 H2O 1.9e-4 7.9e-4
MnCl2 x 4 H2O 9.9e-5 5.0e-4
Pyridoxine hydrochloride 9.9e-5 4.8e-4
ZnCl2 6.9e-5 5.1e-4
Nicotinic acid 4.9e-5 4.0e-4
p-aminobenzoic acid 4.9e-5 3.6e-4
Lipoic acid 4.9e-5 2.4e-4
Thiamine-HCl 4.9e-5 1.5e-4
Riboflavin 4.9e-5 1.3e-4
D-Ca-pantothenate 4.9e-5 1.0e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.0e-4
Biotin 2.0e-5 8.1e-5
Folic acid 2.0e-5 4.5e-5
H3BO3 5.9e-6 9.6e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Vitamin B12 9.9e-7 6.3e-7

Cultivation metadata from 9 strains BacDive

pH 6 - 8 4 strains
pH 8 - 10 3 strains
pH 4 - 6 2 strains
phyla 2 9 strains
nutrition organoheterotroph 1 strain
oxygen anaerobe 7 strains
oxygen obligate anaerobe 1 strain
temperature thermophilic 8 strains
temperature hyperthermophilic 1 strain

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