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507a. CARBOXYDOTHERMUS SIDEROPHILUS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.33 g 0.324 4.34
2 0.52 g 0.51 2.508
3 0.29 g 0.284 1.934
4 0.33 g 0.324 6.048
5 0.33 g 0.324 2.377
6
(AQDS)
8.25 g 8.088 19.617
7 Trace element solution SL-4 10.00 ml - -
8 1.00 g 0.98 11.67
9 0.20 g 0.196 -
10 Vitamin solution 10.00 ml - -
11 1000.00 ml 978.634 54322.4
Instructions:
  1. Dissolve ingredients except bicarbonate, yeast extract, vitamins and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add yeast extract, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. After completing the medium adjust pH to 6.8 - 7.0. Inoculated vessels are pressurized with sterile carbon monoxide gas to 1 bar overpressure.
Solution occurs in 13 other media (first defined in medium 14 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.50 g 0.5 1.487
2 0.20 g 0.2 0.719
3 0.10 g 0.1 0.348
4 0.03 g 0.03 0.152
5 0.30 g 0.3 4.852
6 0.20 g 0.2 0.841
7 0.01 g 0.01 0.059
8 0.02 g 0.02 0.084
9 0.03 g 0.03 0.124
10 1000.00 ml 998.207 55408.8
Instructions:
  1. First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.9
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Na2-9,10-anthraquinone-2,6-disulfonate 8.088 19.617
NaHCO3 0.98 11.67
MgCl2 x 6 H2O 0.51 2.508
NH4Cl 0.324 6.048
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
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