Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KCl | 0.33 | g | 0.326 | 4.378 | |
MgCl2 x 6 H2O | 0.52 | g | 0.514 | 2.53 | |
CaCl2 x 2 H2O | 0.29 | g | 0.287 | 1.951 | |
NH4Cl | 0.33 | g | 0.326 | 6.102 | |
KH2PO4 | 0.33 | g | 0.326 | 2.399 | |
Trace element solution SL-4 | 10.00 | ml | - | - | |
Na2CO3 | 2.50 | g | 2.473 | 23.331 | |
Na-pyruvate | 1.00 | g | 0.989 | 8.988 | |
Yeast extract | 0.20 | g | 0.198 | - | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except carbonate, pyruvate, yeast extract, and vitamins, then sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Add carbonate and adjust pH to 6.0 - 6.5, then dispense medium under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add pyruvate, yeast extract and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Stock solutions of pyruvate and vitamins are sterilized by filtration. After completing the medium adjust pH to 6.5 with a sterile anoxic stock solution of 1 N NaOH. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-EDTA | 0.50 | g | 0.5 | 1.487 | |
FeSO4 x 7 H2O | 0.20 | g | 0.2 | 0.719 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
H3BO3 | 0.30 | g | 0.3 | 4.851 | |
CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 | |
NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
Distilled water | 1000.00 | ml | - | - | |
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 20 | mg | 0.02 | 0.082 |
Folic acid | 20 | mg | 0.02 | 0.045 |
Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
Thiamine HCl | 50 | mg | 0.05 | 0.148 |
Riboflavin | 50 | mg | 0.05 | 0.133 |
Nicotinic acid | 50 | mg | 0.05 | 0.406 |
Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
(DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
Distilled water | 1000 | ml | - | - |
Last modified: | 09.09.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 6.5 |
Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Na2CO3 | 2.473 | 23.331 |
Na-pyruvate | 0.989 | 8.988 |
MgCl2 x 6 H2O | 0.514 | 2.53 |
NH4Cl | 0.326 | 6.102 |
KCl | 0.326 | 4.378 |
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