DSMZ 511 . METHANOPYRUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Gassing station Serum bottle
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.25 g 0.25 4.674
K2HPO4 x 3 H2O 0.07 g 0.07 0.402
KH2PO4 0.09 g 0.09 0.661
NaCl 11.80 g 11.8 201.916
MgSO4 x 7 H2O 1.75 g 1.75 7.1
MgCl2 x 6 H2O 4.50 g 4.5 22.134
Na2SO4 0.81 g 0.81 5.703
CaCl2 x 2 H2O 0.78 g 0.78 5.306
KCl 0.30 g 0.3 4.024
Modified Wolin's mineral solution 10.00 ml - -
Fe(NH4)2(SO4)2 x 6 H2O
(0.1% w/v)
2.00 ml 0.002 -
(NH4)2Ni(SO4)2
(0.1% w/v)
2.00 ml 0.002 0.005
Na2WO4 x 2 H2O
(0.1% w/v)
2.00 ml 0.002 0.006
Marine trace elements solution 10.00 ml - -
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 -
NaHCO3 1.00 g 1 11.904
Wolin's vitamin solution 10.00 ml - -
Na2S x 9 H2O 0.50 g 0.5 2.082
Distilled water 970.00 ml - -
1 Dissolve ingregients except bicarbonate, vitamins and sulfide, then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into serum vials and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins should be sterilized by filtration. Adjust pH of the complete medium to 6.5, if necessary. After inoculation pressurize vessels to 2 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaBr 4.00 g 4 38.877
SrCl2 x 6 H2O 1.80 g 1.8 6.751
H3BO3 1.30 g 1.3 21.022
KI 1.25 mg 0.001 0.005
Na-silicate 100.00 mg 0.1 0.819
NaF 60.00 mg 0.06 1.429
KNO3 40.00 mg 0.04 0.396
Na2HPO4 x 3 H2O 0.25 mg 2.5e-4 0.002
Distilled water 1000.00 ml - -
Solution occurs in 170 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 04.04.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: 6.5
Equipment needed: Autoclave, Filter, Gassing station, Serum bottle
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Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 4.5 22.134
NaHCO3 1 11.904
Na2SO4 0.81 5.703
Na2S x 9 H2O 0.5 2.082
KCl 0.3 4.024
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain