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514. BACTO MARINE BROTH (DIFCO 2216)

Culture medium recipe

Vol.
×
Solution occurs in 2 other media (first defined in medium 514 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 5 -
2 1.00 g 1 -
3 0.10 g 0.1 0.408
4 19.45 g 19.45 332.806
5
(anhydrous)
5.90 g 5.9 61.968
6 3.24 g 3.24 22.81
7 1.80 g 1.8 16.219
8 0.55 g 0.55 7.378
9 0.16 g 0.16 1.905
10 0.08 g 0.08 0.672
11 34.00 mg 0.034 0.214
12 22.00 mg 0.022 0.356
13 4.00 mg 0.004 0.033
14 2.40 mg 0.002 0.057
15 1.60 mg 0.002 0.02
16 8.00 mg 0.008 0.056
17 1000.00 ml 998.207 55408.8
18 , might need strain adjustment 1.00 g/l - -
Instructions:
  1. Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.40 g to 1 litre water.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Taxonomic range:  Archaea
Taxonomic range:  Phages
Medium type: Complex medium
Final pH: 7.6

Associated strains

All strains for this medium: 1914
Strains with modifications: 244
Martelella radicis DSM 28101

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 19.45 332.806
MgCl2 5.9 61.968
Bacto peptone 5 -
Na2SO4 3.24 22.81
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Cultivation metadata from 1914 strains BacDive

halophily moderate 572 strains
halophily not 512 strains
halophily slightly 364 strains
halophily extreme 91 strains
pH 6 - 8 604 strains
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Similar media

1517. MODIFIED BACTO MARINE BROTH (DIFCO 2216) 97 %
1173. MODIFIED MEDIUM 514 97 %
514b. MEDIUM 514 PLUS ADDITIONAL SALT 87 %
1354. BACTO MARINE BROTH WITH NA-ACETATE 87 %
514a. HALF STRENGTH BACTO MARINE BROTH 82 %

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