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514c. REACTIVATION WITH LIQUID MEDIUM 514

Culture medium recipe

Vol.
×

M 514c #

ml
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 5 -
2 1.00 g 1 -
3 0.10 g 0.1 0.408
4 19.45 g 19.45 332.806
5
(dried)
5.90 g 5.9 61.968
6 3.24 g 3.24 22.81
7 1.80 g 1.8 16.219
8 0.55 g 0.55 7.378
9 0.16 g 0.16 1.51
10 0.08 g 0.08 0.672
11 34.00 mg 0.034 0.214
12 22.00 mg 0.022 0.356
13 4.00 mg 0.004 0.033
14 2.40 mg 0.002 0.057
15 1.60 mg 0.002 0.02
16 8.00 mg 0.008 0.056
17
optional
24.00 g 24 -
18 1000.00 ml 998.207 55408.8
Instructions:
  1. Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid medium 514. Subsequent subculturing may be carried out in liquid medium or on agar plates.
  2. Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.4 g to 1 litre water.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.6

Associated strains

All strains for this medium: 70
Strains with modifications: 4
514 BACTO MARINE BROTH (DIFCO 2216)
a HALF STRENGTH BACTO MARINE BROTH
b MEDIUM 514 PLUS ADDITIONAL SALT
c REACTIVATION WITH LIQUID MEDIUM 514
d MODIFIED BACTO MARINE BROTH
e MARINE BROTH (ANAEROB)
f MARINE BROTH (ROTH CP73)

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Agar
optional
24 -
NaCl 19.45 332.806
MgCl2 5.9 61.968
Bacto peptone 5 -
Na2SO4 3.24 22.81
CaCl2 1.8 16.219
Bacto yeast extract 1 -
KCl 0.55 7.378
Na2CO3 0.16 1.51
Fe(III)citrate 0.1 0.408
KBr 0.08 0.672
SrCl2 0.034 0.214
H3BO3 0.022 0.356
Na2HPO4 0.008 0.056
Na-silicate 0.004 0.033
NaF 0.002 0.057
NH4NO3 0.002 0.02

Cultivation metadata from 70 strains BacDive

halophily not 25 strains
halophily moderate 24 strains
halophily slightly 20 strains
pH 4 - 6 21 strains
pH 8 - 10 19 strains
pH 6 - 8 19 strains
pH 10 - 14 13 strains
pH 2 - 4 1 strain
phyla 4 70 strains
oxygen aerobe 24 strains
oxygen facultative anaerobe 12 strains
oxygen facultative aerobe 1 strain
temperature mesophilic 66 strains
temperature psychrophilic 11 strains

Similar media

514b. MEDIUM 514 PLUS ADDITIONAL SALT 95 %
1354. BACTO MARINE BROTH WITH NA-ACETATE 94 %
514f. MARINE BROTH (ROTH CP73) 87 %
514a. HALF STRENGTH BACTO MARINE BROTH 85 %
1517. MODIFIED BACTO MARINE BROTH (DIFCO 2216) 81 %

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