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514c. REACTIVATION WITH LIQUID MEDIUM 514

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 5 -
2 1.00 g 1 -
3 0.10 g 0.1 0.408
4 19.45 g 19.45 332.806
5
(dried)
5.90 g 5.9 61.968
6 3.24 g 3.24 22.81
7 1.80 g 1.8 16.219
8 0.55 g 0.55 7.378
9 0.16 g 0.16 1.51
10 0.08 g 0.08 0.672
11 34.00 mg 0.034 0.214
12 22.00 mg 0.022 0.356
13 4.00 mg 0.004 0.033
14 2.40 mg 0.002 0.057
15 1.60 mg 0.002 0.02
16 8.00 mg 0.008 0.056
17
optional
24.00 g 24 -
18 1000.00 ml 998.207 55408.8
Instructions:
  1. Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid medium 514. Subsequent subculturing may be carried out in liquid medium or on agar plates.
  2. Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.4 g to 1 litre water.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.6

Associated strains

All strains for this medium: 70
Strains with modifications: 4
Fangia hongkongensis DSM 21703

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Agar
optional
24 -
NaCl 19.45 332.806
MgCl2 5.9 61.968
Bacto peptone 5 -
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Cultivation metadata from 70 strains BacDive

halophily not 25 strains
halophily moderate 24 strains
halophily slightly 20 strains
pH 4 - 6 21 strains
pH 8 - 10 19 strains
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Similar media

514b. MEDIUM 514 PLUS ADDITIONAL SALT 95 %
1354. BACTO MARINE BROTH WITH NA-ACETATE 94 %
514f. MARINE BROTH (ROTH CP73) 87 %
514a. HALF STRENGTH BACTO MARINE BROTH 85 %
1517. MODIFIED BACTO MARINE BROTH (DIFCO 2216) 81 %

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