Name: REACTIVATION WITH LIQUID MEDIUM 514
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 514c #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
514. BACTO MARINE BROTH (DIFCO 2216)	1000	ml	-	-
1 Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid medium 514. Subsequent subculturing may be carried out in liquid medium or on agar plates.

514. BACTO MARINE BROTH (DIFCO 2216) #

1000

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Bacto peptone	5.00	g	5	-
Yeast extract (Bacto )	1.00	g	1	-
Fe(III) citrate	0.10	g	0.1	0.408
NaCl	19.45	g	19.45	332.82
MgCl₂ (dried)	5.90	g	5.9	61.968
Na₂SO₄	3.24	g	3.24	22.811
CaCl₂	1.80	g	1.8	16.219
KCl	0.55	g	0.55	7.378
Na₂CO₃	0.16	g	0.16	1.51
KBr	0.08	g	0.08	0.672
SrCl2	34.00	mg	0.034	0.214
H₃BO₃	22.00	mg	0.022	0.356
Na-silicate	4.00	mg	0.004	0.033
NaF	2.40	mg	0.002	0.057
(NH₄)NO₃	1.60	mg	0.002	0.02
Na₂HPO₄	8.00	mg	0.008	0.056
Agar	18.00	g	18	53.519
Distilled water	1000.00	ml	-	-
1 Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.4 g to 1 litre water.

All strains for this medium:	70
Strains with modifications:	4

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123	MARINE AGAR
514	BACTO MARINE BROTH (DIFCO 2216)
a	HALF STRENGTH BACTO MARINE BROTH
b	MEDIUM 514 plus additional salt
c	REACTIVATION WITH LIQUID MEDIUM 514
d	MODIFIED BACTO MARINE BROTH
e	MARINE BROTH (ANAEROB)
f	MARINE BROTH (ROTH CP73)
g	HALF STRENGTH BACTO MARINE BROTH with 50% Seawater
760	MARINE THERMOCOCCUS MEDIUM
1173	MODIFIED MEDIUM 514