514c .
REACTIVATION WITH LIQUID MEDIUM 514
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Equipment needed:
Final pH:
7.6
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
514. BACTO MARINE BROTH (DIFCO 2216) | 1000 | ml | ||
| 1 Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid medium 514. Subsequent subculturing may be carried out in liquid medium or on agar plates. | |||||
514. BACTO MARINE BROTH (DIFCO 2216) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Bacto peptone | 5.00 | g | ||
|
|
Yeast extract
(Bacto ) |
1.00 | g | ||
|
|
Fe(III) citrate | 0.10 | g | ||
|
|
NaCl | 19.45 | g | ||
|
|
MgCl2
(dried) |
5.90 | g | ||
|
|
Na2SO4 | 3.24 | g | ||
|
|
CaCl2 | 1.80 | g | ||
|
|
KCl | 0.55 | g | ||
|
|
Na2CO3 | 0.16 | g | ||
|
|
KBr | 0.08 | g | ||
|
|
SrCl2 | 34.00 | mg | ||
|
|
H3BO3 | 22.00 | mg | ||
|
|
Na-silicate | 4.00 | mg | ||
|
|
NaF | 2.40 | mg | ||
|
|
(NH4)NO3 | 1.60 | mg | ||
|
|
Na2HPO4 | 8.00 | mg | ||
|
|
Agar | 18.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.4 g to 1 litre water. | |||||