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517. DESULFOBACTERIUM OLEOVORANS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 920 ml - -
2 Solution B 1 ml - -
3 Solution C 1 ml - -
4 Solution D 30 ml - -
5 Solution E 10 ml - -
6 Solution F 1 ml - -
7 Solution G 11 ml - -
8 Solution H 10 ml - -
9 , may stimulate growth of some strains 20 mg/l 0.02 -
Instructions:
  1. Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 45 min), then distributed under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclaved.
  2. Solutions B, C, G, and H are autoclaved separately under 100% N2 gas. Solution D is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions E and F are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to H are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.1 - 7.4, if necessary.
  3. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5%(w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.20 g 0.217 1.597
2 0.25 g 0.272 5.08
3 4.00 g 4.348 30.609
4 20.00 g 21.739 371.975
5 3.40 g 3.696 18.178
6 0.25 g 0.272 1.848
7
(0.1% w/v)
0.50 ml 5.4e-4 0.002
8 920.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace element solution SL-10 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Selenite-tungstate solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.5 g 50 471.752
2 30.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Vitamin solution 10 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(50 μg/ml)
1 ml 2700 1709.32
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.36 g 32.727 115.043
2
(2 N )
0.63 ml 121.991 3049.99
3 10.00 ml 907.461 50371.7
Instructions:
  1. Heat the suspension in a closed bottle (with 100% N2 gas atmosphere in head space) in a boiling water bath. Shake until stearate has dissolved, then autoclave. Stored stearate solution has to be remelted before use.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 40 166.54
2 10.0 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.4
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 996.168 55295.6
NaCl 20.325 347.781
Na2SO4 4.065 28.618
MgCl2 x 6 H2O 3.455 16.996
Vitamin B12 2.744 1.737
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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