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520. RUMINOCLOSTRIDIUM CELLULOLYTICUM (CM3) MEDIUM

Culture medium recipe

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* Modified for Acetivibrio aldrichii DSM 6159
beta

Anaerobic

Equipment needed: Autoclave Filter Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.30 g 1.296 9.809
2 1.50 g 1.496 10.99
3 2.90 g 2.891 12.669
4
(0.1% w/v in 0.1 N H2SO4)
1.25 ml 0.001 0.004
5 Trace element solution SL-10 1.00 ml - -
6 2.00 g 1.994 -
7
(0.1% w/v)
0.50 ml 5.0e-4 0.002
8 0.20 g 0.199 0.981
9 75.00 mg 0.075 0.509
10 6.00 g 5.982 17.476
11 1.50 g 1.496 14.11
12 0.50 g 0.499 2.838
13 1000.00 ml 995.221 55243.1
16 , some strains can be adapted to cellulose
(powder MN 301 MACHEREY-NAGEL)
10.00 g/l 10 -
Instructions:
  1. Dissolve ingredients except magnesium chloride, calcium chloride, cellobiose, cysteine and carbonate, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add magnesium chloride, calcium chloride and cellobiose from anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2. Cellobiose has to be sterilized by filtration. Prior to inoculation add cysteine from a sterile anoxic stock solution prepared under 100% N2 gas and adjust pH to 7.2.
  2. Note: Some strains can be adapted to cellulose as substrate using 10.00 g/l cellulose powder MN 301 (MACHEREY-NAGEL).
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 14
Strains with modifications: 14
Acetivibrio aldrichii DSM 6159

Gas composition

  • 100 % N2

Light

Intensity lux

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 996.206 55297.8
Cellulose 10 -
Cellobiose 5.982 17.476
K2HPO4 x 3 H2O 2.891 12.669
Yeast extract 1.994 -
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Cultivation metadata from 14 strains BacDive

halophily not 1 strain
pH 6 - 8 3 strains
pH 8 - 10 2 strains
pH 4 - 6 2 strains
phyla 1 14 strains
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