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DSMZ

DSMZ 523 . CLOSTRIDIUM CHARTATABIDUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 523 #

ml
Equipment needed: Autoclave Hungate tubes Filter Gassing station Centrifuge
Compound Amount Unit Conc. [g/L] Conc. [mM]
Mineral solution 38.00 ml - -
Clarified rumen fluid 200.00 ml - -
K2HPO4 0.30 g 0.3 1.722
Trypticase peptone
(BD BBL) 		2.00 g 2 -
Yeast extract
(OXOID) 		0.50 g 0.5 -
Haemin solution
(0.05% w/v) 		2.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 -
Na2CO3 4.00 g 4 37.74
Cellobiose 5.00 g 5 14.607
L-Cysteine HCl x H2O 0.25 g 0.25 1.423
Na2S x 9 H2O 0.25 g 0.25 1.041
Distilled water 760.00 ml - -
1 Dissolve ingredients (except carbonate, cellobiose, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 100% CO2 gas atmosphere. Add the carbonate and equilibrate the medium with the CO2 gas to pH 6.8. Distribute under CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cellobiose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Cellobiose has to be sterilized by filtration. Adjust pH of complete medium to 6.7 - 6.8, if necessary.

Mineral solution #

1000
Solution occurs in 4 other media (first defined in Medium 330).
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 6.0 g 6 44.09
NaCl 12.0 g 12 205.339
(NH4)2SO4 6.0 g 6 45.406
CaCl2 x 2 H2O 1.6 g 1.6 10.883
MgSO4 x 7 H2O 2.5 g 2.5 10.143
Distilled water 1000.0 ml - -

Clarified rumen fluid #

1000
Solution occurs in 28 other media (first defined in Medium 1310).
1 Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 min and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min, dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C.

Haemin solution #

100
Solution occurs in 15 other media (first defined in Medium 78).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Haemin 50 mg 0.5 0.767
NaOH
(1 N) 		1 ml - -
Distilled water 100 ml - -
1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.

Metadata

Last modified: 04.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.7 - 6.8
Equipment needed: Autoclave, Centrifuge, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Cellobiose 5 14.607
Na2CO3 4 37.74
Trypticase peptone 2 -
Yeast extract 0.5 -
NaCl 0.456 7.803
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains