Name: FLEXISTIPES MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 524 #

Equipment needed: Autoclave Hungate tubes Gassing station

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	58.35	g	58.35	998.46
MgCl₂ x 6 H₂O	37.80	g	37.8	185.929
Na₂SO₄	9.72	g	9.72	68.431
CaCl₂ x 2 H₂O	7.14	g	7.14	48.567
KCl	1.65	g	1.65	22.133
KBr	0.24	g	0.24	2.017
SrCl2 x 6 H₂O	0.17	g	0.17	0.638
H₃BO₃	0.66	g	0.66	10.673
Na₂HPO₄ x 2 H₂O	0.03	g	0.03	0.211
Na-meta silicate	12.00	mg	0.012	-
NaF (0.1% w/v)	7.00	ml	0.007	0.167
Sodium resazurin (0.1% w/v)	0.50	ml	5.0e-4	-
NaHCO₃	0.50	g	0.5	5.952
Yeast extract	1.00	g	1	-
KNO₃	0.25	g	0.25	2.473
Na₂S x 9 H₂O	0.50	g	0.5	2.082
Distilled water	1000.00	ml	-	-
1 Dissolve ingredients except bicarbonate, yeast extract, nitrate and sulfide, adjust pH to 6.0 - 6.2 using sulfuric acid and sparge medium with 100% N₂ gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Complete medium by adding yeast extract, nitrate and sulfide from sterile anoxic stock solutions prepared under 100% N₂ gas atmosphere and bicarbonate from a sterile anoxic stock solution prepared under 80% N₂ and 20% CO₂. Adjust pH of complete medium to 6.5.

Last modified:	04.04.22
Source:	DSMZ
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	6.5
Equipment needed:	Autoclave, Gassing station, Hungate tubes

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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

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524
524 . FLEXISTIPES MEDIUM