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525. METHANOHALOPHILUS (MG) MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Hungate tubes Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.34 g 0.333 4.471
2 2.75 g 2.696 13.261
3 3.45 g 3.382 13.723
4 0.25 g 0.245 4.582
5 0.14 g 0.137 0.601
6 0.14 g 0.137 0.934
7 100.00 g 98.039 1677.53
8 Trace element solution 10.00 ml - -
9 1.00 g 0.98 11.951
10
(0.1% w/v)
0.50 ml 4.9e-4 0.002
11 4.00 g 3.922 46.682
12 0.50 g 0.49 4.625
13 5.00 g 4.902 51.291
14 Vitamin solution 10.00 ml - -
15 0.50 g 0.49 2.791
16 0.50 g 0.49 2.041
17 1000.00 ml 978.634 54322.4
Instructions:
  1. Dissolve ingredients except carbonates, trimethylamine, vitamins, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Complete medium by adding sterile anoxic stock solutions of trimethylamine, vitamins (sterilized by filtration), cysteine and sulfide prepared under 100% N2 gas atmosphere and bicarbonate and carbonate from sterile anoxic stock solutions prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 6.9 - 7.0, if necessary.
Solution occurs in 192 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.172
3 0.50 g 0.5 2.958
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.18 g 0.18 0.64
7 0.10 g 0.1 0.68
8 0.18 g 0.18 0.626
9 0.01 g 0.01 0.04
10 0.02 g 0.02 0.042
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 0.03 g 0.03 0.126
14 0.30 mg 3.0e-4 0.001
15 0.40 mg 4.0e-4 0.001
16 1000.00 ml 998.207 55408.8
Instructions:
  1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 98.049 1677.7
Trimethylamine hydrochloride 4.902 51.291
NaHCO3 3.922 46.682
MgSO4 x 7 H2O 3.412 13.842
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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