MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 525a . METHANOHALOPHILUS (MG+NaCl) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 525a #

ml
Equipment needed: Autoclave Filter Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.34 g 0.336 4.511
MgCl2 x 6 H2O 2.75 g 2.72 13.379
MgSO4 x 7 H2O 3.45 g 3.412 13.845
NH4Cl 0.25 g 0.247 4.623
K2HPO4 x 3 H2O 0.14 g 0.138 0.795
CaCl2 x 2 H2O 0.14 g 0.138 0.942
NaCl 150.00 g 148.368 2538.81
Modified Wolin's mineral solution 10.00 ml - -
Na-acetate 1.00 g 0.989 12.058
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
NaHCO3 4.00 g 3.956 47.097
Na2CO3 0.50 g 0.495 4.666
Trimethylamine-HCl 5.00 g 4.946 -
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O 0.50 g 0.495 2.816
Na2S x 9 H2O 0.50 g 0.495 2.059
Distilled water 1000.00 ml - -
1 Dissolve ingredients except carbonates, trimethylamine, vitamins, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Complete medium by adding sterile anoxic stock solutions of trimethylamine, vitamins (sterilized by filtration), cysteine and sulfide prepared under 100% N2 gas atmosphere and bicarbonate and carbonate from sterile anoxic stock solutions prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 6.9 - 7.0, if necessary.

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 04.04.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: 6.9 - 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
Edit in Medium builder

Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 5

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trimethylamine-HCl 4.946 -
NaHCO3 3.956 47.097
MgCl2 x 6 H2O 2.72 13.379
Na-acetate 0.989 12.058
Na2CO3 0.495 4.666
Show more
CSV
JSON

Cultivation metadata from 5 strains BacDive

phyla 1 5 strains
oxygen anaerobe 5 strains
temperature mesophilic 5 strains