526
526 .
CLOSTRIDIUM VIRIDE MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 526 #
ml
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| 5-Aminovaleric acid | 15.00 | g | 15 | 128.041 | |
| K2HPO4 | 8.70 | g | 8.7 | 49.95 | |
| Yeast extract | 7.50 | g | 7.5 | - | |
| Trace elements solution VR | 4.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| NaHCO3 | 2.50 | g | 2.5 | 29.759 | |
| L-Cysteine HCl x H2O | 0.25 | g | 0.25 | 1.423 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except bicarbonate and cysteine), adjust pH to 7.0 with NaOH and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min. Add and dissolve solid bicarbonate and cysteine, dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving adjust pH of medium to 7.6 - 7.8 using a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
5.00 | g | 5 | 26.159 | |
| MgSO4 x 7 H2O | 24.00 | g | 24 | 97.373 | |
| CaCl2 x 2 H2O | 0.50 | g | 0.5 | 3.401 | |
| FeSO4 x 7 H2O | 0.50 | g | 0.5 | 1.798 | |
| NiCl2 x 6 H2O | 0.25 | g | 0.25 | 1.929 | |
| ZnSO4 x 7 H2O | 0.44 | g | 0.44 | 1.53 | |
| MnSO4 x H2O | 0.25 | g | 0.25 | 1.479 | |
| CoCl2 x 6 H2O | 0.25 | g | 0.25 | 1.925 | |
| VOSO4 x 5 H2O | 0.25 | g | 0.25 | 0.988 | |
| Na2MoO4 x 2 H2O | 0.25 | g | 0.25 | 1.033 | |
| CuSO4 x 5 H2O | 0.12 | g | 0.12 | 0.481 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve mineral salts in 700 ml water acidified with 2 ml of conc. HCl. Separately dissolve 5.00 g of NTA in 300 ml water add 10 N NaOH until all the NTA has dissolved and the pH is 7.0. While stirring, slowly add the NTA solution to the salt solution. Stir until the solution is almost clear. Store cool and dark. | |||||
Metadata
| Last modified: | 04.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.6 - 7.8 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| 5-Aminovaleric acid | 15 | 128.041 |
| K2HPO4 | 8.7 | 49.95 |
| Yeast extract | 7.5 | - |
| NaHCO3 | 2.5 | 29.759 |
| L-Cysteine HCl x H2O | 0.25 | 1.423 |
| Show more | ||
