529
529 .
AMPHIBACILLUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Amphibacillus cookii DSM 23721
This strain uses the standard medium without modifications.
Growth conditions: facultative anaerobic
37 °C
Main sol. 529 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Glucose | 10.0 | g | 10 | 55.506 | |
| K2HPO4 | 1.0 | g | 1 | 5.741 | |
| NH4NO3 | 2.0 | g | 2 | - | |
| MgSO4 x 7 H2O | 0.2 | g | 0.2 | 0.811 | |
| MnSO4 x 7 H2O | 5.0 | mg | 0.005 | 0.033 | |
| FeSO4 x 7 H2O | 5.0 | mg | 0.005 | 0.018 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| Yeast extract | 3.0 | g | 3 | - | |
|
Polypeptone
(BBL) |
0.3 | g | 0.3 | - | |
| Agar | 15.0 | g | 15 | 44.599 | |
| Distilled water | 900.0 | ml | - | - | |
| Na-sesquicarbonate solution | 100.0 | ml | - | - | |
| 1 After sterilization add sterile 1 M Na-sesquicarbonate solution (1 ml in 10 ml) to achieve a pH of 9.7 (see medium 31). | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaHCO3 | 4.2 | g | 42 | 499.958 |
|
Na2CO3 anhydrous
(anhydrous) |
5.3 | g | 53 | 500.057 |
| Distilled water | 100.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.7 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 2 |
Amphibacillus cookii DSM 23721
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Glucose | 10 | 55.506 |
| Na2CO3 anhydrous | 5.3 | 50.006 |
| NaHCO3 | 4.2 | 49.996 |
| Yeast extract | 3 | - |
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