530 .
THIOGLYCOLATE MEDIUM
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Selected strain: Bacillus sp. DSM 5538
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Equipment needed:
Autoclave
Filter
Final pH:
5.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casein peptone | 5.0 | g | ||
|
|
Meat extract | 3.0 | g | ||
|
|
Na-thioglycolate | 0.5 | g | ||
|
|
Agar | 15.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 5.5. | |||||
| 2 Sterilize Na-Thioglycolate separately by filtration. | |||||
| 3 Optimal pO2 for growth on solid media is 0.03 - 0.08 atm. | |||||