533
533 .
THERMOPHILIC HYDROGEN BACTERIA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 533 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4NO3 | 1.0 | g | 1 | - | |
| Na2HPO4 x 12 H2O | 4.5 | g | 4.5 | 31.699 | |
| KH2PO4 | 1.5 | g | 1.5 | 11.023 | |
| MgSO4 x 7 H2O | 0.2 | g | 0.2 | 0.811 | |
| FeSO4 x 7 H2O | 10.0 | mg | 0.01 | 0.036 | |
| CaCl2 x 2 H2O | 10.0 | mg | 0.01 | 0.068 | |
| NaCl | 1.0 | g | 1 | 17.112 | |
| Trace element solution | 0.5 | ml | - | - | |
|
H2O
(deionized) |
1000.0 | ml | - | - | |
| 1 pH 7.0 | |||||
| 2 Incubate (e.g., in a serum bottle) with a headspace of 5% (v/v) O2, 85% H2, 10% CO2. | |||||
Solution occurs in 2
other media (first defined in Medium 533).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
H2O
(deionized) |
1000 | ml | - | - |
| MoO3 | 4 | mg | 0.004 | 0.028 |
| ZnSO4 x 7 H2O | 28 | mg | 0.028 | 0.097 |
| CuSO4 x 5 H2O | 2 | mg | 0.002 | 0.008 |
| H3BO3 | 4 | mg | 0.004 | 0.065 |
| MnSO4 x 5 H2O | 4 | mg | 0.004 | 0.017 |
| CoCl2 x 6 H2O | 4 | mg | 0.004 | 0.031 |
Metadata
| Last modified: | 22.03.24 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.0 |
| Edit in Medium builder | |
Gas composition
- 80 % H2
- 10 % CO2
- 5 % O2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2HPO4 x 12 H2O | 4.5 | 31.699 |
| KH2PO4 | 1.5 | 11.023 |
| NaCl | 1 | 17.112 |
| NH4NO3 | 1 | - |
| MgSO4 x 7 H2O | 0.2 | 0.811 |
| Show more | ||
