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DSMZ

DSMZ 534 . MODIFIED SME MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 534 #

ml
Solution occurs in 1 other media (first defined in Medium 534).
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 30 513.347
MgSO4 x 7 H2O 7.00 g 7 28.4
MgCl2 x 6 H2O 5.50 g 5.5 27.053
KCl 0.65 g 0.65 8.719
NaBr 0.10 g 0.1 0.972
NH4Cl 0.15 g 0.15 2.804
K2HPO4 0.15 g 0.15 0.861
CaCl2 x 2 H2O 0.50 g 0.5 3.401
Trace element solution 10.00 ml - -
Distilled water 1000.00 ml - -
1 Sparge medium with 100% N2 gas mixture for 20 min to make it anoxic.
2 Adjust pH to 6.5-6.8 with 1N H2SO4.
3 Fill 10 ml of medium in serum bottles (100 ml) under a protective H2/CO2 atmosphere and tightly stopper.
4 Autoclave at 121 °C for 15 min.
5 after autoclaving add the following filter-sterilized solutions.
Solution A , 0.3 ml/10ml
(gassed with CO2) 		30.00 ml/l - -
Solution B , 0.3 ml/10ml
(gassed with N2) 		30.00 ml/l - -
6 apply xx bar H2/CO2 (80%/20%) + yy % O2 (Note: strain specific conditions need to be applied)
7 For more details on the medium see the original description (Huber et al. System. Appl. Microbiol. 15:340-351 (1992)).

Trace element solution #

1000
Solution occurs in 1 other media (first defined in Medium 534).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Modified Wolin's mineral solution II 1000 ml - -
1 To 1000.0 ml trace element solution in medium 141 add:
(NH4)2Ni(SO4)2 2 g 2 5.063
Na2WO4 10 mg 0.01 0.034
Na2SeO4 10 mg 0.01 0.053

Solution A #

30
Solution occurs in 1 other media (first defined in Medium ).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 2 g 66.667 793.585
Distilled water 30 ml - -
1 prepared under CO2 in an ice-bath, filter-sterilise

Solution B #

30
Solution occurs in 1 other media (first defined in Medium ).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 2 g 66.667 268.616
Distilled water 30 ml - -
1 Prepared under N2,autoclave 121°C, 15 min

Modified Wolin's mineral solution II #

1000
Solution occurs in 24 other media (first defined in Medium 700).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
MgSO4 x 7 H2O 3.000 g 3 12.172
MnSO4 x H2O 0.500 g 0.5 2.958
NaCl 1.000 g 1 17.112
FeSO4 x 7 H2O 0.100 g 0.1 0.36
CoSO4 x 7 H2O 0.180 g 0.18 1.161
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnSO4 x 7 H2O 0.180 g 0.18 0.626
CuSO4 x 5 H2O 0.010 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.020 g 0.02 0.042
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.025 g 0.025 0.193
Na2SeO3 x 5 H2O 0.300 mg 3.0e-4 0.001
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 11.01.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.6
Equipment needed: Autoclave, Filter
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Gas composition

  • 80 % H2
  • 20 % CO2
  • 0.5 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 4
Strains with modifications: 4

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 5.5 27.053
NaHCO3 2 23.808
Na2S2O3 x 5 H2O 2 8.058
KCl 0.65 8.719
NH4Cl 0.15 2.804
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Cultivation metadata from 4 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 3 strains
nutrition chemolithoautotroph 1 strain
temperature hyperthermophilic 2 strains
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