DSMZ 535 . TRYPTICASE SOY BROTH AGAR

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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Selected strain: Nocardioides simplex DSM 20130

This strain uses the standard medium without modifications.

Solution occurs in 18 other media (first defined in Medium 535).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase soy broth
(BBL 11768, Oxoid CM129 or Merck 5459)
30 g - -
Agar 20 g - -
Distilled water 1000 ml - -
1 pH 7.3
2 Autoclave at 121°C for 15 min.

Metadata

Last modified: 19.09.22
Source: DSMZ
Synonyms: Tryptic Soy Agar, Tryptic Soy Broth Agar, TSA
Taxonomic range:  Bacteria
Taxonomic range:  Phages
Medium type: Complex medium
Final pH: 7.3
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1535
Strains with modifications: 64
Nocardioides simplex DSM 20130

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trypticase soy broth 30 -
Agar 15 44.599
Distilled water - -

Cultivation metadata from 1535 strains BacDive

halophily halotolerant 4 strains
halophily non-halophilic 1 strain
pH 6 - 8 206 strains
pH 8 - 10 131 strains
pH 4 - 6 118 strains
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