535a
535a .
TRYPTICASE SOY BROTH AGAR+BLOOD
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
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Medium for Corynebacterium glutamicum DSM 20411
This strain uses the standard medium without modifications.
Growth conditions: 28 °C
Main sol. 535a #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium 535 | 1000 | ml | - | - | |
| Sheep blood | 5 | % | 50 | - | |
| 1 To medium 535 add 5% blood after cooling. | |||||
Solution occurs in 18
other media (first defined in Medium 535).
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase soy broth
(BBL 11768, Oxoid CM129 or Merck 5459) |
30 | g | - | - | |
| Agar | 20 | g | - | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 pH 7.3 | |||||
| 2 Autoclave at 121°C for 15 min. | |||||
Metadata
| Last modified: | 19.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 89 |
| Strains with modifications: | 1 |
Corynebacterium glutamicum DSM 20411
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| 50 | - | |
| Trypticase soy broth | 30 | - |
| Agar | 15 | 44.599 |
| Distilled water | - | - |
