536
536 .
THIOCAPSA HALOPHILA MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 536 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.30 | g | 0.3 | 2.205 | |
| NH4Cl | 0.50 | g | 0.5 | 9.348 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.34 | |
| NaCl | 70.00 | g | 70 | 1197.81 | |
| MgCl2 x 6 H2O | 4.50 | g | 4.5 | 22.134 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| Trace element solution SL-12 B | 1.00 | ml | - | - | |
| H2O | 1000.00 | ml | - | - | |
| 1 To the cold medium add following filter-sterilized solutions: | |||||
|
Vitamin B12
(2.0 mg in 100 ml H2O) |
1.00 | ml | - | - | |
|
NaHCO3
(5.0 g in 100 ml H2O) |
40.00 | ml | - | - | |
|
Na2S x 9 H2O
(6.0 g in 100 ml H2O) |
10.00 | ml | - | - | |
|
Na2S2O3
(5.0 g in 100 ml H2O) |
10.00 | ml | - | - | |
| 2 Autoclave at 121°C for 15 min and then cool to under N2/CO2 (9:1). | |||||
| 3 Adjust final pH 7.2 to 7.3. | |||||
Equipment needed: Ice bath
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2-EDTA | 3.0 | g | 3 | 8.923 | |
| FeSO4 x 7 H2O | 1.1 | g | 1.1 | 3.957 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| MnCl2 x 2 H2O | 50.0 | mg | 0.05 | - | |
| ZnCl2 | 42.0 | mg | 0.042 | 0.308 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 18.0 | mg | 0.018 | 0.074 | |
| H3BO3 | 300.0 | mg | 0.3 | 4.851 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 6.0. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Medium type: | Defined medium |
| Final pH: | 7.2 - 7.3 |
| Equipment needed: | Autoclave, Filter, Ice bath |
| Edit in Medium builder | |
Gas composition
- 90 % N2
- 10 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 70 | 1197.81 |
| MgCl2 x 6 H2O | 4.5 | 22.134 |
| MgSO4 x 7 H2O | 3 | 12.172 |
| NH4Cl | 0.5 | 9.348 |
| KH2PO4 | 0.3 | 2.205 |
| Show more | ||
Cultivation metadata from 0 strains
| phyla | 1 | 1 strain |