539
539 .
RUMINICLOSTRIDIUM CELLOBIOPARUM MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 539 #
ml
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.20 | g | 0.2 | 1.468 | |
| NH4Cl | 0.30 | g | 0.3 | 5.603 | |
| NaCl | 1.00 | g | 0.999 | 17.095 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.4 | 1.966 | |
| KCl | 0.50 | g | 0.5 | 6.7 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.15 | 1.019 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Yeast extract | 0.50 | g | 0.5 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 1.00 | g | 0.999 | 9.426 | |
| Cellobiose | 5.00 | g | 4.995 | 14.593 | |
|
Cellulose
, MN 301
optional |
5.00 | g | 4.995 | - | |
| Na2S x 9 H2O | 0.40 | g | 0.4 | 1.664 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, cellobiose and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add cellobiose (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 7.0, if necessary. | |||||
| 2 Note: Some strains can be adapted to cellulose as substrate using 5.00 g/l cellulose powder MN 301 (MACHEREY-NAGEL). | |||||
Solution occurs in 265
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Metadata
| Last modified: | 04.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Cellobiose | 4.995 | 14.593 |
|
Cellulose optional |
4.995 | - |
| NaCl | 0.999 | 17.095 |
| Na2CO3 | 0.999 | 9.426 |
| KCl | 0.5 | 6.7 |
| Show more | ||
