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DSMZ

DSMZ 541 . SULFUROSPIRILLUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 541 #

ml
Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 952 ml - -
Solution B 30 ml - -
Solution C 20 ml - -
Solution D 2 ml - -
1 Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C should be prepared under 100% N2 gas and sterilized by filtration. Solution D is autoclaved under 100% N2 gas atmosphere. To complete the medium appropriate amounts of solutions B to D are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.2, if necessary.

Solution A #

952
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.36 g 1.429 10.498
MgSO4 x 7 H2O 0.37 g 0.389 1.577
CaCl2 x 2 H2O 0.10 g 0.105 0.714
NH4Cl 0.27 g 0.284 5.302
Trace element solution SL-10 2.00 ml - -
Distilled water 950.00 ml - -

Solution B #

30
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1.5 g 50 471.752
Distilled water 30.0 ml - -

Solution C #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-fumarate 4 g 200 1249.72
Distilled water 20 ml - -

Solution D #

2
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Cysteine HCl x H2O 0.07 g 35 199.276
Distilled water 2.00 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 19.08.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2-fumarate 3.984 24.895
Na2CO3 1.494 14.096
KH2PO4 1.355 9.954
MgSO4 x 7 H2O 0.369 1.495
NH4Cl 0.269 5.028
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Cultivation metadata from 3 strains BacDive

pH 6 - 8 1 strain
phyla 1 3 strains
oxygen anaerobe 2 strains
oxygen facultative anaerobe 1 strain
temperature mesophilic 3 strains