54a
54a .
REACTIVATION WITH LIQUID MEDIUM 545
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 54a #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| 54. GLUCOSE YEAST EXTRACT AGAR | 1000 | ml | - | - | |
| 545. TRYPTONE SOYA BROTH (TSB) | 1000 | ml | - | - | |
| 1 Rehydrate and grow lyophilized cells from the ampoule in liquid medium 54 or 545. Subsequent subculturing may be carried out in liquid medium or on agar plates medium 54. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Glucose | 20 | g | 20 | 111.012 |
| Yeast extract | 10 | g | 10 | - |
|
CaCO3
(light precipitate) |
20 | g | 20 | 199.82 |
| Agar , for solid medium | 17 | g | 17 | 50.546 |
| Distilled water | 1000 | ml | - | - |
Solution occurs in 4
other media (first defined in Medium 54a).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casein peptone | 17.0 | g | 17 | - | |
| Soy peptone | 3.0 | g | 3 | - | |
| D(+)-Glucose | 2.5 | g | 2.5 | 13.877 | |
| NaCl | 5.0 | g | 5 | 85.558 | |
| K2HPO4 | 2.5 | g | 2.5 | 14.353 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.3. | |||||
| 2 The same as Caso Bouillon (Merck or Oxoid, Germany). | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.3 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 9 |
