DSMZ 54a . REACTIVATION WITH LIQUID MEDIUM 545

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Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
54. GLUCOSE YEAST EXTRACT AGAR 1000 ml - -
545. TRYPTONE SOYA BROTH (TSB) 1000 ml - -
1 Rehydrate and grow lyophilized cells from the ampoule in liquid medium 54 or 545. Subsequent subculturing may be carried out in liquid medium or on agar plates medium 54.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 20 g 20 111.012
Yeast extract 10 g 10 -
CaCO3
(light precipitate)
20 g 20 199.82
Agar , for solid medium 17 g 17 50.546
Distilled water 1000 ml - -
Solution occurs in 4 other media (first defined in Medium 54a).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 17.0 g 17 -
Soy peptone 3.0 g 3 -
D(+)-Glucose 2.5 g 2.5 13.877
NaCl 5.0 g 5 85.558
K2HPO4 2.5 g 2.5 14.353
Distilled water 1000.0 ml - -
1 Adjust pH to 7.3.
2 The same as Caso Bouillon (Merck or Oxoid, Germany).

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3
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Associated strains

All strains for this medium: 9

Cultivation metadata from 9 strains BacDive

phyla 1 9 strains
temperature mesophilic 9 strains