54a .
REACTIVATION WITH LIQUID MEDIUM 545
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Equipment needed:
Final pH:
7.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
54. GLUCOSE YEAST EXTRACT AGAR | 1000 | ml | ||
|
|
545. TRYPTONE SOYA BROTH (TSB) | 1000 | ml | ||
| 1 Rehydrate and grow lyophilized cells from the ampoule in liquid medium 54 or 545. Subsequent subculturing may be carried out in liquid medium or on agar plates medium 54. | |||||
54. GLUCOSE YEAST EXTRACT AGAR #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Glucose | 20 | g |
|
|
Yeast extract | 10 | g |
|
|
CaCO3
(light precipitate) |
20 | g |
|
|
Agar , for solid medium | 17 | g |
|
|
Distilled water | 1000 | ml |
545. TRYPTONE SOYA BROTH (TSB) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casein peptone | 17.0 | g | ||
|
|
Soy peptone | 3.0 | g | ||
|
|
D(+)-Glucose | 2.5 | g | ||
|
|
NaCl | 5.0 | g | ||
|
|
K2HPO4 | 2.5 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 7.3. | |||||
| 2 The same as Caso Bouillon (Merck or Oxoid, Germany). | |||||