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54b. CLOSTRIDIUM SP. MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 20 g 20 111.015
2 10 g 10 -
3
(light precipitate)
20 g 20 199.826
4
optional
17 g 17 -
5 1000 ml 998.207 55408.8
Instructions:
  1. Dissolve ingredients (note that most of the carbonate remains suspended) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials vials while stirring to keep carbonate suspended and autoclave.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown
Equipment needed: Autoclave, Hungate tubes

Associated strains

All strains for this medium: 8
Strains with modifications: 8

Gas composition

  • 100 % N2

Light

8 strains need light for growth. Select a strain for more details.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
CaCO3 20 199.826
Glucose 20 111.015
Agar
optional
17 -
Yeast extract 10 -

Cultivation metadata from 8 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 8 strains
oxygen anaerobe 7 strains
temperature mesophilic 8 strains
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