Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Solution A | 500.0 | ml | - | - | |
Solution B | 300.0 | ml | - | - | |
Solution C | 200.0 | ml | - | - | |
Agar , if required | 1.5 | % | 15 | 44.599 | |
1 Autoclave solutions A and B separately, filter sterilize solution C. Add 1.5% agar if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Na2HPO4 x 12 H2O | 9.0 | g | 18 | 126.797 |
KH2PO4 | 1.5 | g | 3 | 22.045 |
NH4Cl | 1.0 | g | 2 | 37.39 |
MgSO4 x 7 H2O | 0.2 | g | 0.4 | 1.623 |
Trace element solution SL-6 | 0.3 | ml | - | - |
Distilled water | 500.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
CaCl2 x 2 H2O | 0.01 | g | 0.033 | 0.227 |
Ferric ammonium citrate | 5.00 | mg | 0.017 | 0.064 |
Distilled water | 300.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Fructose | 5 | g | 25 | 138.766 |
Distilled water | 200 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 |
MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 |
H3BO3 | 0.30 | g | 0.3 | 4.851 |
CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 |
CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 |
NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 |
Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 |
Distilled water | 1000.00 | ml | - | - |
Last modified: | 15.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | n.d. |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 3 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Agar | 15 | 44.599 |
Na2HPO4 x 12 H2O | 9 | 63.399 |
Fructose | 5 | 27.753 |
KH2PO4 | 1.5 | 11.023 |
NH4Cl | 1 | 18.695 |
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