Loading ...

557. CLOSTRIDIUM SP. MEDIUM (CREATININE)

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.05 g 0.05 0.293
2 0.50 g 0.504 2.045
3 0.01 g 0.01 0.036
4 0.06 g 0.06 0.306
5 1.00 g 1.008 17.249
6 Trace element solution SL-11 1.00 ml - -
7 Selenite-tungstate solution 1.00 ml - -
8 5.00 g 5.04 -
9
(0.1% w/v)
0.50 ml 5.0e-4 0.002
10 2.64 g 2.661 19.556
11 5.33 g 5.373 30.848
12 1.00 g 1.008 12
13 5.50 g 5.544 49.014
14 Vitamin solution 10.00 ml - -
15 0.50 g 0.504 2.87
16 0.50 g 0.504 2.099
17 980.00 ml 986.132 54738.6
18 , might need strain adjustment
(SIGMA)
6.80 g/l - -
Instructions:
  1. Dissolve ingredients except hydrogenphosphates, bicarbonate, creatinine, vitamins, cysteine and sulfide, adjust pH to 7.0 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Complete medium by adding hydrogenphosphates, creatinine, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Stock solutions of creatinine (5% w/v) and vitamins should be sterilized by filtration. Adjust pH of complete medium to 7.0, if necessary.
Solution occurs in 22 other media (first defined in medium 722 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.2 g 5.2 13.97
2 1.5 g 1.5 7.545
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 24.0 mg 0.024 0.101
9 36.0 mg 0.036 0.149
10 1000.0 ml 998.207 55408.8
Instructions:
  1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 2
Strains with modifications: 2

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.9
Creatinine 5.544 49.014
K2HPO4 5.373 30.848
Yeast extract 5.04 -
KH2PO4 2.661 19.556
Show more

Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains

Similar media

771. SULFUROSPIRILLUM BARNESII MEDIUM 80 %
120a. METHANOSARCINA BARKERI MEDIUM 79 %
311. SPOROMUSA MEDIUM (BETAINE) 79 %
487. PEPTOCLOSTRIDIUM MEDIUM (BETAINE) 78 %
500. CLOSTRIDIUM (GV) MEDIUM 78 %

Latest comments

No comments for this medium.