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DSMZ

DSMZ 559 . TMBS4 MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 559 #

ml
Equipment needed: Gassing station Autoclave Hungate tubes Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 932 ml - -
Solution B 30 ml - -
Solution C 10 ml - -
Solution D 2 ml - -
Solution E 10 ml - -
Solution F 10 ml - -
Solution G 10 ml - -
1 Boil solution A for 3 min., then cool to room temperature under 100% N2 gas atmosphere. Dispense the medium under same gas atmosphere in culture vessels and autoclave. Autoclave separately solutions E and F under 100% N2 and solution B under 80% N2 and 20% CO2 gas atmosphere. Solutions C, D, and G are prepared under N2 gas atmosphere and sterilized by filtration.
2 Solutions B to G are added to the sterile, cooled solution A in the sequence as indicated. The final pH of the medium should be 7.2-7.4. After inoculation add 25 mg sodium dithionite per liter medium from a 2.5% (w/v) solution, freshly prepared under N2 and filter-sterilized.

Solution A #

932
Equipment needed: Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.00 g 1.073 18.36
MgCl2 x 6 H2O 0.40 g 0.429 2.111
CaCl2 x 2 H2O 0.15 g 0.161 1.095
KCl 0.50 g 0.536 7.196
NH4Cl 0.25 g 0.268 5.015
KH2PO4 0.20 g 0.215 1.577
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Resazurin 0.50 mg 5.4e-4 0.002
Distilled water 930.00 ml - -

Solution B #

30
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 , 5% w/v solution 30 ml - -

Solution C #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin solution 10 ml - -

Solution D #

2
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-thiosulfate , 5% w/v solution 2 ml 1667 10543.3

Solution E #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract , 10% w/v solution 10 ml - -

Solution F #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Syringate solution 6.0 %(w/v) 10 ml - -

Solution G #

10
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Dithiothreitol , 100 mM solution 10 ml - -

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2 mg 0.002 0.008
Folic acid 2 mg 0.002 0.005
Pyridoxine hydrochloride 10 mg 0.01 0.049
Thiamine HCl 5 mg 0.005 0.015
Riboflavin 5 mg 0.005 0.013
Nicotinic acid 5 mg 0.005 0.041
Calcium D-(+)-pantothenate 5 mg 0.005 -
Vitamin B12 5 mg 0.005 0.004
p-Aminobenzoic acid 5 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5 mg 0.005 0.024
Distilled water 1000 ml - -
1 Adjust final pH of solution to 7.2 using 50 mM sodium phosphate buffer.

Syringate solution 6.0 %(w/v) #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Syringic acid 6 %(w/v) 60 -
1 Dissolve syringic acid in distilled water and neutralize solution with 2 N NaOH until a pH of 7.5 is reached. The final concentration of syringate in the solution should be 6% w/v.

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Solution occurs in 178 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Metadata

Last modified: 28.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2 - 7.4
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na-thiosulfate 3.321 21.003
NaCl 0.996 17.043
Syringic acid 0.598 -
KCl 0.498 6.68
MgCl2 x 6 H2O 0.398 1.96
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain