DSMZ 573 . THIOTHRIX MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.20 g 0.2 3.739
K2HPO4 0.01 g 0.01 0.057
MgSO4 x 7 H2O 0.01 g 0.01 0.041
CaSO4
(saturated solution)
20.00 ml - -
Trace element solution 5.00 ml - -
Na-acetate 0.10 g 0.1 1.219
Agar , if required 12.00 g 12 35.679
Distilled water 1000.00 ml - -
1 Adjust pH to 7.5 before autoclaving. Sterilize separately a 10% (w/v) solution of Na2S x 9 H2O; add the following amount of this solution to the medium after autoclaving (= shortly before using the medium).
neutralized Na2S x 9 H2O solution, 10% (w/v) 3.00 ml - -
2 Note: For small volumes, such as 5 mL agar slants, you can use the respective volume of a sterile neutralized 3% Na2S x 9 H2O solution (e.g., 50µL/5 mL = 0.3g/L)
Solution occurs in 1 other media (first defined in Medium 155).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.20 g 0.2 0.684
FeSO4 x 7 H2O 0.70 g 0.7 2.518
ZnSO4 x 7 H2O 0.01 g 0.01 0.035
MnSO4 x 4 H2O 2.00 mg 0.002 0.009
CuSO4 x 5 H2O 5.00 µg 5.0e-6 2.0e-5
H3BO3 10.00 mg 0.01 0.162
Co(NO3)2 1.00 mg 1.0e-3 0.005
Na2MoO4 x 2 H2O 1.00 mg 1.0e-3 0.004
Distilled water 1000.00 ml - -
Solution occurs in 1 other media (first defined in Medium ).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 3 %(w/v) 30 124.905
Distilled water 1000 ml - -
1 Adjust pH to 7.0.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 10 g - -
Distilled water 100 ml - -
1 Adjust pH to 7.0.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5
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Associated strains

All strains for this medium: 3
Strains with modifications: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 12 35.679
Na2S x 9 H2O 0.3 1.249
NH4Cl 0.2 3.739
Na-acetate 0.1 1.219
K2HPO4 0.01 0.057
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Cultivation metadata from 3 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 2 strains
oxygen aerobe 1 strain
temperature mesophilic 1 strain
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