Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
NH4Cl | 0.20 | g | ||
|
K2HPO4 | 0.01 | g | ||
|
MgSO4 x 7 H2O | 0.01 | g | ||
|
CaSO4
(saturated solution) |
20.00 | ml | ||
|
Trace element solution | 5.00 | ml | ||
|
Na-acetate | 0.10 | g | ||
|
Agar , if required | 12.00 | g | ||
|
Distilled water | 1000.00 | ml | ||
1 Adjust pH to 7.5 before autoclaving. Sterilize separately a 10% (w/v) solution of Na2S x 9 H2O; add the following amount of this solution to the medium after autoclaving (= shortly before using the medium). | |||||
|
neutralized Na2S x 9 H2O solution, 10% (w/v) | 3.00 | ml | ||
2 Note: For small volumes, such as 5 mL agar slants, you can use the respective volume of a sterile neutralized 3% Na2S x 9 H2O solution (e.g., 50µL/5 mL = 0.3g/L) |
Compound | Amount | Unit | |
---|---|---|---|
|
EDTA | 0.20 | g |
|
FeSO4 x 7 H2O | 0.70 | g |
|
ZnSO4 x 7 H2O | 0.01 | g |
|
MnSO4 x 4 H2O | 2.00 | mg |
|
CuSO4 x 5 H2O | 5.00 | µg |
|
H3BO3 | 10.00 | mg |
|
Co(NO3)2 | 1.00 | mg |
|
Na2MoO4 x 2 H2O | 1.00 | mg |
|
Distilled water | 1000.00 | ml |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Na2S x 9 H2O | 3 | %(w/v) | ||
|
Distilled water | 1000 | ml | ||
1 Adjust pH to 7.0. |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Na2S x 9 H2O | 10 | g | ||
|
Distilled water | 100 | ml | ||
1 Adjust pH to 7.0. |