576
576 .
SUCCINATE MINERAL MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 576 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Succinate | 10.00 | mM | 1.161 | 10 | |
| KH2PO4 | 0.39 | g | 0.39 | 2.866 | |
| K2HPO4 x 3 H2O | 1.63 | g | 1.63 | 9.358 | |
| MgSO4 x 7 H2O | 62.00 | mg | 0.062 | 0.252 | |
| NH4Cl | 1.02 | g | 1.02 | 19.069 | |
| Trace element solution | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 pH 6.8 | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Solution A | 1000 | ml | - | - | |
| Solution B | 1000 | ml | - | - | |
| 1 Autoclave trace element solutions A and B separately and mix identical volumes: | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CaCl2 x 2 H2O | 140 | mg | 0.14 | 0.952 |
| Na2-EDTA | 250 | mg | 0.25 | 0.744 |
| FeCl2 x 4 H2O | 140 | mg | 0.14 | 0.704 |
| Distilled water | 1000 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CoCl2 x 2 H2O | 28.00 | mg | 0.028 | 0.216 |
| CuCl2 x 2 H2O | 0.70 | mg | 7.0e-4 | 0.004 |
| Na2-EDTA | 250.00 | mg | 0.25 | 0.744 |
| H3BO3 | 30.00 | mg | 0.03 | 0.485 |
| MnCl2 x 4 H2O | 4.00 | mg | 0.004 | 0.02 |
| Na2MoO4 x 2 H2O | 3.00 | mg | 0.003 | 0.012 |
| NiCl2 x 6 H2O | 1.65 | mg | 0.002 | 0.013 |
| ZnCl2 | 5.00 | mg | 0.005 | 0.037 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Medium type: | Defined medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |