582 .
15% MH MEDIUM
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Equipment needed:
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 121.500 | g | ||
|
|
MgCl2 | 10.500 | g | ||
|
|
MgSO4 | 14.400 | g | ||
|
|
CaCl2 | 0.540 | g | ||
|
|
KCl | 3.000 | g | ||
|
|
NaHCO3 | 0.090 | g | ||
|
|
NaBr | 0.039 | g | ||
|
|
Proteose peptone no. 3
(Difco) |
5.000 | g | ||
|
|
Yeast extract
(Difco) |
10.000 | g | ||
|
|
Glucose | 1.000 | g | ||
|
|
Distilled water | 1000.000 | ml | ||
| 1 Final pH 7.5 | |||||
| 2 NaHCO3 is added from a sterilized stock solution when the medium has cooled. Agar is added at a concentration of 20 g/l for solid media. | |||||