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585. BCYE-AGAR

Culture medium recipe

Vol.
×

M 585 #

ml
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 490 ml - -
2 Solution B 490 ml - -
3 Solution C 10 ml - -
4 Solution D 10 ml - -
Instructions:
  1. Dehydrated media preparations are available from media distributors.
  2. We use OXOID Legionella CYE- Agar base with Legionella BCYE supplement.
  3. For solubilization of the lyophilized pellet use standard liquid media. Although Legionellae do not grow in those media they are suitable for resuspension and transfer onto agar plates.
  4. Alternatively use:
  5. Add Cysteine- HCl, then ferric pyrophosphate solution to solution A. Adjust the complete medium to pH 6.9 ± 0.5 at 50°C with sterile 1.0 N KOH or HCl. The pH of the medium is critical. Finally, add solution B. Swirl medium in flask during dispensing to petri dishes or tubes in order to keep charcoal suspended.
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(Difco 0127)
10 g 20.408 -
2
(N-2-acetamido-2-aminoethane-sulfonic acid)
10 g 20.408 -
3
(Sigma C 5510)
2 g 4.082 -
4 490 ml 998.207 55408.8
5 , required for pH adjustment 1 N - -
Instructions:
  1. Dissolve ACES and bring to pH 6.9 by slowly adding 1 N KOH. Add the other components and dissolve by boiling. Autoclave at 121°C for 15 minutes. Cool to 50 - 55°C.
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 15 g 30.612 -
2 490 ml 998.207 55408.8
Instructions:
  1. Autoclave at 121°C and cool to 50 - 55°C.
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 40 227.744
2 10.0 ml 998.207 55408.8
Instructions:
  1. Prepare a fresh solution and filter-sterilize it.
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.25 g 25 -
2 10.00 ml 998.207 55408.8
Instructions:
  1. Prepare a fresh solution by heating to 50 - 55°C in a water-bath. Filter-sterilize solution separately.
  2. Please note: Soluble ferric pyrophosphate must be kept dry and in the dark. Do not use if chemical looses its green colour and becomes brown or yellow.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.9
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 107
Strains with modifications: 91

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Agar 15 -
ACES 10 -
Yeast extract 10 -
Activated charcoal 2 -
L-Cysteine HCl x H2O 0.4 2.277
Fe4(PO4)2 0.25 -
KOH - -

Cultivation metadata from 107 strains BacDive

halophily not 1 strain
halophily moderate 1 strain
pH 8 - 10 2 strains
pH 6 - 8 2 strains
pH 4 - 6 2 strains
pH 10 - 14 1 strain
phyla 1 107 strains
oxygen microaerophile 82 strains
oxygen aerobe 8 strains
oxygen facultative aerobe 1 strain
temperature mesophilic 107 strains
temperature thermophilic 3 strains
temperature psychrophilic 1 strain

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