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DSMZ

DSMZ 585 . BCYE AGAR

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Legionella worsleiensis DSM 21907

* Modification: Microaerophilic; needs high humidity

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 3-7 days 37 °C

Main sol. 585 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
OXOID Legionella CYE-Agar base
(with Legionella BCYE supplement) 		1000 ml - -
1 Dehydrated media preparations are available from media distributors.
2 We use OXOID Legionella CYE-Agar base with Legionella BCYE supplement.
3 For solubilization of the lyophilized pellet use standard liquid media. Although Legionellae do not grow in those media they are suitable for resuspension and transfer onto agar plates.
4 Note: Alternatively use CYE-ACES AGAR

CYE-ACES AGAR #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 490 ml - -
Solution B 490 ml - -
Solution C 10 ml - -
Solution D 10 ml - -
1 Add Cysteine-HCl, then ferric pyrophosphate solution to solution A. Adjust the complete medium to pH 6.9 ± 0.5 at 50°C with sterile 1.0 N KOH or HCl. The pH of the medium is critical. Finally, add solution B. Swirl medium in flask during dispensing to petri dishes or tubes in order to keep charcoal suspended.

Solution A #

490
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(Difco 0127) 		10 g 20.408 -
ACES
(N-2-acetamido-2-aminoethane-sulfonic acid) 		10 g 20.408 112.01
Activated charcoal
(Sigma C 5510) 		2 g 4.082 -
Distilled water 490 ml - -
1 Dissolve ACES and bring to pH 6.9 by slowly adding 1 N KOH. Add the other components and dissolve by boiling. Autoclave at 121°C for 15 minutes. Cool to 50 - 55°C.

Solution B #

490
Solution occurs in 1 other media (first defined in Medium 585).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Agar 15 g 30.612 -
Distilled water 490 ml - -
1 Autoclave at 121°C and cool to 50 - 55°C.

Solution C #

10
Solution occurs in 1 other media (first defined in Medium 585).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Cysteine HCl x H2O 0.4 g 40 227.744
Distilled water 10.0 ml - -
1 Prepare a fresh solution and filter-sterilize it.

Solution D #

10
Solution occurs in 1 other media (first defined in Medium 585).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe4(PO4)2 0.25 g 25 33.548
Distilled water 10.00 ml - -
1 Prepare a fresh solution by heating to 50 - 55°C in a water-bath. Filter-sterilize solution separately.
2 Please note: Soluble ferric pyrophosphate must be kept dry and in the dark. Do not use if chemical looses its green colour and becomes brown or yellow.

Metadata

Last modified: 20.12.21
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 111
Strains with modifications: 90
Legionella worsleiensis DSM 21907

Molecular composition

Compound Conc. [g/L] Conc. [mM]
OXOID Legionella CYE-Agar base - -
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Cultivation metadata from 111 strains BacDive

pH 4 - 6 4 strains
pH 6 - 8 3 strains
phyla 1 115 strains
oxygen microaerophile 86 strains
oxygen aerobe 10 strains
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