DSMZ 58 . BIFIDOBACTERIUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Selected strain: Bifidobacterium reuteri DSM 23975

This strain uses the standard medium without modifications.

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone
(tryptic digest)
10.00 g 10 -
Yeast extract 5.00 g 5 -
Meat extract 5.00 g 5 -
Bacto Soytone 5.00 g 5 -
Glucose 10.00 g 10 55.506
K2HPO4 2.00 g 2 11.483
MgSO4 x 7 H2O 0.20 g 0.2 0.811
MnSO4 x H2O 0.05 g 0.05 0.296
Tween 80 1.00 ml - -
NaCl 5.00 g 5 85.558
L-Cysteine HCl x H2O 0.50 g 0.5 -
Salt solution 40.00 ml - -
Resazurin
(25 mg/100ml)
4.00 ml - -
Distilled water 950.00 ml - -
1 The cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 6.8 using 8 N NaOH. Distribute under N2 and autoclave.
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.25 g 0.25 1.701
MgSO4 x 7 H2O 0.50 g 0.5 2.029
K2HPO4 1.00 g 1 5.741
KH2PO4 1.00 g 1 7.348
NaHCO3 10.00 g 10 119.038
NaCl 2.00 g 2 34.223
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave
Edit in Medium builder

Associated strains

All strains for this medium: 201
Strains with modifications: 18
Bifidobacterium reuteri DSM 23975

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 10 55.506
Casein peptone 10 -
Bacto Soytone 5 -
Meat extract 5 -
Yeast extract 5 -
Show more

Cultivation metadata from 201 strains BacDive

pH 6 - 8 18 strains
pH 4 - 6 12 strains
pH 8 - 10 3 strains
pH 2 - 4 2 strains
pH 10 - 12 1 strain
Show more