DSMZ 591 . HALOBACTEROIDES/HALOINCOLA MEDIUM

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Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.33 6.169
CaCl2 0.33 g 0.33 2.974
MgCl2 0.33 g 0.33 3.466
KCl 0.33 g 0.33 4.427
KH2PO4 0.33 g 0.33 2.425
NaCl , adjust if required in range 100-150 125.00 g 125 2138.95
NaHCO3 1.50 g 1.5 17.856
Na2S x 9 H2O 0.50 g 0.5 2.082
Glucose 5.00 g 5 27.753
Peptone
(Bacto Proteose Peptone No.3)
5.00 g 5 -
Trace element solution SL-10
(SL 10)
1.00 ml - -
Wolin's vitamin solution 10.00 ml - -
Resazurin 2.00 mg 0.002 0.009
Distilled water 990.00 ml - -
1 Prepare the medium without NaHCO3, Na2S, glucose and vitamins, boil and cool under a nitrogen:carbon dioxide (80:20 v/v) atmosphere. Glucose (filter-sterilized and stored under nitrogen), vitamins (filter sterilized and stored under nitrogen), and sodium sulphide (autoclaved under nitrogen) are added from sterile stock solutions. The pH should be pH 7.5.
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 9
Strains with modifications: 8

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 125 2138.95
Glucose 5 27.753
Peptone 5 -
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.5 2.082
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Cultivation metadata from 9 strains BacDive

phyla 1 9 strains
oxygen anaerobe 8 strains
temperature mesophilic 8 strains
temperature thermophilic 1 strain