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591. HALOBACTEROIDES/HALOINCOLA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.33 g 0.33 6.163
2 0.33 g 0.33 2.97
3 0.33 g 0.33 3.463
4 0.33 g 0.33 4.422
5 0.33 g 0.33 2.423
6 , adjust if required 175.00 g 174.825 2991.4
7 1.50 g 1.499 17.838
8 0.50 g 0.5 2.08
9 5.00 g 4.995 27.726
10
(Bacto Proteose Peptone No.3)
5.00 g 4.995 -
11 Trace element solution SL-10 1.00 ml - -
12 Vitamin solution 10.00 ml - -
13 2.00 mg 0.002 0.009
14 990.00 ml 987.238 54800
15 , might need strain adjustment
(Oxoid)
0.50 g/l - -
Instructions:
  1. Prepare the medium without NaHCO3, Na2S, glucose and vitamins, boil and cool under a nitrogen:carbon dioxide (80:20 v/v) atmosphere. Glucose (filter-sterilized and stored under nitrogen), vitamins (filter sterilized and stored under nitrogen), and sodium sulphide (autoclaved under nitrogen) are added from sterile stock solutions. The pH should be pH 7.5.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 9
Strains with modifications: 9

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.3
NaCl 174.825 2991.4
Glucose 4.995 27.726
Peptone 4.995 -
NaHCO3 1.499 17.838
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Cultivation metadata from 9 strains BacDive

phyla 1 9 strains
oxygen anaerobe 8 strains
temperature mesophilic 8 strains
temperature thermophilic 1 strain

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