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DSMZ

DSMZ 60 . MOORELLA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 60 #

ml
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 843 ml - -
Solution B 50 ml - -
Solution C 50 ml - -
Solution D 40 ml - -
Solution E 10 ml - -
Solution F 10 ml - -
1 Sparge solution A with 100% CO2 gas for 30 - 45 min to make it anoxic, then distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Autoclave separately solutions B, C, E and F under 100% N2 gas, and solution D under 80% N2 and 20% CO2 gas atmosphere. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Adjust pH of the complete medium to 6.9, if necessary.

Solution A #

843
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 0.10 g 0.119 0.481
(NH4)2SO4 0.50 g 0.593 4.489
Fe(NH4)2(SO4)2 0.04 g 0.047 -
Na2MoO4 x 2 H2O
(0.1% w/v) 		2.40 ml 0.003 0.012
Na2SeO3 x 5 H2O
(0.1% w/v) 		0.15 ml 1.8e-4 6.8e-4
Tryptone 5.00 g 5.931 -
Yeast extract 5.00 g 5.931 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.9e-4 0.002
Distilled water 840.00 ml - -

Solution B #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 7.0 g 140 803.785
KH2PO4 4.5 g 90 661.351
Distilled water 50.0 ml - -

Solution C #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 18 g 360 1998.22
Distilled water 50 ml - -

Solution D #

40
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 2 g 50 471.752
Distilled water 40 ml - -

Solution E #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Cysteine HCl x H2O 0.3 g 30 170.808
Distilled water 10.0 ml - -

Solution F #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.3 g 30 124.905
Distilled water 10.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.9
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 4

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 17.946 99.612
K2HPO4 6.979 40.069
Yeast extract 4.985 -
Tryptone 4.985 -
KH2PO4 4.487 32.969
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Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
oxygen anaerobe 4 strains
temperature thermophilic 4 strains