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603. ANCALOMICROBIUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.250 g 0.248 1.873
2 0.250 g 0.248 1.374
3 Vitamin solution 10.000 ml - -
4 Hutner's salts 20.000 ml - -
5 0.071 g 0.07 0.495
6 980.000 ml 968.557 53763
7 , might need strain adjustment 15.000 g/l - -
8 , might need strain adjustment 0.100 g/l - -
Instructions:
  1. pH 7
  2. Make up the medium without the vitamin solution and autoclave it. The filter-sterilized vitamin solution is added to the cooled medium. The medium may be solidified by adding 15 g/l agar.
Solution occurs in 3 other media (first defined in medium 603 )
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.1 mg 1.0e-4 6.3e-5
2 2.0 mg 0.002 0.008
3 5.0 mg 0.005 0.013
4 5.0 mg 0.005 0.01
5 2.0 mg 0.002 0.005
6 5.0 mg 0.005 0.013
7 5.0 mg 0.005 0.041
8 1000.0 ml 998.207 55408.8
Instructions:
  1. Filter-sterilized
Solution occurs in 25 other media (first defined in medium 1327 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(NTA)
10.000 g 10 52.318
2 29.700 g 29.7 120.499
3 3.335 g 3.335 22.685
4 9.250 mg 0.009 0.007
5 99.000 mg 0.099 0.356
6 "Metals 44" 50.000 ml - -
7 950.000 ml 948.297 52638.4
Instructions:
  1. Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.

"Metals 44" #

1000
Solution occurs in 37 other media (first defined in medium 1137 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 250.0 mg 0.25 0.744
2 1095.0 mg 1.095 3.808
3 500.0 mg 0.5 1.798
4 154.0 mg 0.154 0.911
5 39.2 mg 0.039 0.157
6 24.8 mg 0.025 0.085
7 17.7 mg 0.018 0.046
8 1000.0 ml 998.207 55408.8
9 , required for dissolving EDTA x - -
Instructions:
  1. Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 5
Strains with modifications: 3
Ancalomicrobium adetum DSM 4722

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
MgSO4 x 7 H2O 0.588 2.386
(NH4)2SO4 0.248 1.873
Glucose 0.248 1.374
Nitrilotriacetic acid 0.198 1.036
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Cultivation metadata from 5 strains BacDive

halophily not 1 strain
pH 6 - 8 2 strains
phyla 2 5 strains
nutrition chemoorganotroph 2 strains
oxygen microaerophile 1 strain
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