607a
607a .
FILOBACILLUS MILOSENSIS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 607a #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 0.75 | g | 0.75 | - | |
| Yeast extract | 0.75 | g | 0.75 | - | |
| Glucose | 0.75 | g | 0.75 | 4.163 | |
| Vitamin solution | 10.00 | ml | - | - | |
| Hutner's salts | 20.00 | ml | - | - | |
|
Tris-HCl buffer
(0.1M, pH 7.5) |
50.00 | ml | - | 5 | |
| Artifical sea water | 250.00 | ml | - | - | |
| Distilled water | 670.00 | ml | - | - | |
| NaCl | 10.00 | %(w/v) | 100 | 1711.16 | |
| 1 Autoclave at 121°C for 15 min. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
10.000 | g | 10 | 52.318 | |
| MgSO4 x 7 H2O | 29.700 | g | 29.7 | 120.499 | |
| CaCl2 x 2 H2O | 3.335 | g | 3.335 | 22.685 | |
| (NH4)6Mo7O24 x 4 H2O | 9.250 | mg | 0.009 | - | |
| FeSO4 x 7 H2O | 99.000 | mg | 0.099 | 0.356 | |
| Distilled water | 950.000 | ml | - | - | |
| "Metals 44" | 50.000 | ml | - | - | |
| 1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4. | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 | |
| Biotin | 2.0 | mg | 0.002 | 0.008 | |
| Thiamine-HCl x 2 H2O | 5.0 | mg | 0.005 | 0.013 | |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 | |
| Folic acid | 2.0 | mg | 0.002 | 0.005 | |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 | |
| Nicotinamide | 5.0 | mg | 0.005 | 0.041 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Filter-sterilized | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaCl | 23.477 | g | 23.477 | 401.728 |
| Na2SO4 | 3.917 | g | 3.917 | 27.577 |
| MgCl2 x 6 H2O | 4.981 | g | 4.981 | 24.5 |
| CaCl2 | 1.102 | g | 1.102 | 9.93 |
| NaHCO3 | 192.000 | mg | 0.192 | 2.286 |
| KCl | 664.000 | mg | 0.664 | 8.907 |
| KBr | 6.000 | mg | 0.006 | 0.05 |
| H3BO3 | 26.000 | mg | 0.026 | 0.42 |
| SrCl2 | 24.000 | mg | 0.024 | 0.151 |
| NaF | 3.000 | mg | 0.003 | 0.071 |
| Distilled water | 1000.000 | ml | - | - |
Solution occurs in 34
other media (first defined in Medium 590).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na-EDTA | 250.0 | mg | 0.25 | 0.744 | |
| ZnSO4 x 7 H2O | 1095.0 | mg | 1.095 | 3.808 | |
| FeSO4 x 7 H2O | 500.0 | mg | 0.5 | 1.798 | |
| MnSO4 x H2O | 154.0 | mg | 0.154 | 0.911 | |
| CuSO4 x 5 H2O | 39.2 | mg | 0.039 | 0.157 | |
| Co(NO3)2 x 6 H2O | 24.8 | mg | 0.025 | 0.085 | |
| Na2B4O7 x 10 H2O | 17.7 | mg | 0.018 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. | |||||
Metadata
| Last modified: | 23.03.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 5.869 | 100.432 |
| MgCl2 x 6 H2O | 1.245 | 6.125 |
| Na2SO4 | 0.979 | 6.894 |
| Glucose | 0.75 | 4.163 |
| Peptone | 0.75 | - |
| Show more | ||
