612 .
DESULFOSARCINA CETONICA MEDIUM
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Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final pH:
7.2 - 7.4
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 962 | ml | ||
|
|
Solution B | 20 | ml | ||
|
|
Solution C | 10 | ml | ||
|
|
Solution D | 10 | ml | ||
| 1 Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclaved. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solutions C and D are autoclaved under 100% N2 gas atmosphere. To complete the medium appropriate amounts of solutions B to D are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.2 – 7.4. | |||||
Solution A #
962
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
KH2PO4 | 0.70 | g |
|
|
NH4Cl | 0.30 | g |
|
|
Na2SO4 | 2.80 | g |
|
|
MgCl2 x 6 H2O | 1.70 | g |
|
|
CaCl2 x 2 H2O | 0.05 | g |
|
|
NaCl | 10.00 | g |
|
|
Trace element solution SL-10 | 1.00 | ml |
|
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml |
|
|
Distilled water | 960.00 | ml |
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||