DSMZ 613 . THERMOTOGA TF(C) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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* Modified for Thermotoga neapolitana DSM 4359

Replace glucose with 0.50 g/l soluble starch added to the autoclaved medium from a sterile anoxic stock solution prepared under 100% N2 gas.

Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.50 g 0.495 9.246
K2HPO4 1.60 g 1.583 9.086
Na2HPO4 x H2O 1.00 g 0.989 6.968
NaCl 7.00 g 6.924 118.478
MgCl2 x 6 H2O 1.40 g 1.385 6.811
MgSO4 x 7 H2O 1.96 g 1.939 7.866
Modified Wolin's mineral solution 10.00 ml - -
Yeast extract
(OXOID)
2.00 g 1.978 -
Trypticase peptone
(BD BBL)
2.00 g 1.978 -
Sodium resazurin
(0.1% w/v)
0.50 ml 4.9e-4 0.002
D-Glucose* 3.00 g 2.967 16.471
CaCl2 x 2 H2O 0.06 g 0.059 0.404
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O 0.30 g 0.297 1.689
Na2S x 9 H2O 0.30 g 0.297 1.235
Starch* 0.50 g - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except glucose, calcium chloride, vitamins and reducing agents), adjust pH to 6.5 - 6.8 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add glucose, calcium chloride, vitamins (sterilized by filtration), sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere.
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 299 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 09.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5 - 6.8
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 1
Thermotoga neapolitana DSM 4359

  D-Glucose
  0.5 g Starch

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Yeast extract 1.978 -
Trypticase peptone 1.978 -
K2HPO4 1.583 9.086
MgCl2 x 6 H2O 1.385 6.811
Na2HPO4 x H2O 0.989 6.968
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature hyperthermophilic 1 strain
temperature thermophilic 1 strain