615
615 .
DESULFOVIBRIO (MG-1) MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 615 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.00 | g | 0.996 | 18.621 | |
| KH2PO4 | 0.50 | g | 0.498 | 3.66 | |
| Na2SO4 | 4.50 | g | 4.482 | 31.555 | |
| CaCl2 x 2 H2O | 0.04 | g | 0.04 | 0.271 | |
| MgSO4 x 7 H2O | 0.06 | g | 0.06 | 0.242 | |
| Glycerol | 2.00 | g | 1.992 | 21.631 | |
| Yeast extract | 1.00 | g | 0.996 | - | |
| Trisodium citrate x 2 H2O | 0.60 | g | 0.598 | 2.032 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
|
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) |
4.00 | ml | 0.004 | 0.014 | |
| Na-thioglycolate | 0.10 | g | 0.1 | 0.873 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients, adjust pH to 7.5 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. | |||||
Metadata
| Last modified: | 09.09.22 |
| Source: | DSMZ |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .