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DSMZ

DSMZ 616 . ALKALITHERMOBACTER MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Thermobrachium celere DSM 8682

* Modification: Reduce amount of carbonate to 4.40 g/l and adjust pH of complete medium to 9.0.

Growth conditions: anaerobic 55 °C

Main sol. 616 #

ml
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 0.36 g 0.36 2.536
KCl 0.08 g 0.08 1.073
Yeast extract
(OXOID) 		5.00 g 5 -
Tryptone
(BD Bacto) 		10.00 g 10 140.687
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 -
Na2CO3* 4.40 g - -
D-Glucose 3.00 g 3 16.652
L-Cysteine HCl x H2O 0.20 g 0.2 1.139
Na2S x 9 H2O 0.20 g 0.2 0.833
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate, glucose, cysteine and sulfide) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonate, dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add glucose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and adjust pH to 10 by adding sterile anoxic 3 N NaOH.

Metadata

Last modified: 12.02.24
Source: DSMZ
Synonyms: CLOSTRIDIUM (YTG) MEDIUM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: * 9.0
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 4
Strains with modifications: 1
Thermobrachium celere DSM 8682

  9.0
  Na2CO3
  4.4 g Na2CO3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tryptone 10 140.687
Na2CO3 5.3 50.006
Yeast extract 5 -
D-Glucose 3 16.652
Na2HPO4 x 2 H2O 0.36 2.536
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Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
oxygen anaerobe 4 strains
temperature thermophilic 4 strains