Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
This strain uses the standard medium without modifications.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2HPO4 x 2 H2O | 0.36 | g | 0.36 | 2.536 | |
KCl | 0.08 | g | 0.08 | 1.073 | |
Yeast extract
(OXOID) |
5.00 | g | 5 | - | |
Tryptone
(BD Bacto) |
10.00 | g | 10 | 140.687 | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
Na2CO3 | 5.30 | g | 5.3 | 50.006 | |
D-Glucose | 3.00 | g | 3 | 16.652 | |
L-Cysteine HCl x H2O | 0.20 | g | 0.2 | 1.139 | |
Na2S x 9 H2O | 0.20 | g | 0.2 | 0.833 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except carbonate, glucose, cysteine and sulfide) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonate, dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add glucose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and adjust pH to 10 by adding sterile anoxic 3 N NaOH. |
Last modified: | 12.02.24 |
Source: | DSMZ |
Synonyms: | CLOSTRIDIUM (YTG) MEDIUM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 10.0 |
Equipment needed: | Autoclave, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 4 |
Strains with modifications: | 1 |
No modifications necessary for this strain.
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Tryptone | 10 | 140.687 |
Na2CO3 | 5.3 | 50.006 |
Yeast extract | 5 | - |
D-Glucose | 3 | 16.652 |
Na2HPO4 x 2 H2O | 0.36 | 2.536 |
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