61
61 .
THERMOANAEROBACTER MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 61 #
ml
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Tryptone
(BD BBL) |
10.00 | g | 10 | - | |
| Yeast extract | 2.00 | g | 2 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| FeSO4 x 7 H2O | 0.20 | g | 0.2 | 0.719 | |
| Sucrose | 10.00 | g | 10 | 29.214 | |
| Na2SO3 | 0.20 | g | 0.2 | 1.587 | |
| Na2S2O3 x 5 H2O | 0.08 | g | 0.08 | 0.322 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except ferrous sulfate, sucrose, sulfite, and thiosulfate), adjust pH to 6.8 - 7.5, and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add ferrous sulfate, then dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add sucrose, sulfite, and thiosulfate from anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. The stock solution of sulfite should be freshly prepared. | |||||
Metadata
| Last modified: | 11.01.24 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 - 7.5 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sucrose | 10 | 29.214 |
| Tryptone | 10 | - |
| Yeast extract | 2 | - |
| Na2SO3 | 0.2 | 1.587 |
| FeSO4 x 7 H2O | 0.2 | 0.719 |
| Show more | ||
Cultivation metadata from 17 strains
| phyla | 2 | 17 strains |
| oxygen | anaerobe | 16 strains |
| temperature | thermophilic | 16 strains |
| temperature | mesophilic | 1 strain |